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human l selectin negative molt  (ATCC)


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    ATCC human l selectin negative molt
    Human L Selectin Negative Molt, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+l+selectin+negative+molt/MOLT-3/pmc12355078-305-0-8
    Average 95 stars, based on 286 article reviews
    human l selectin negative molt - by Bioz Stars, 2026-09
    95/100 stars

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    Transduction:

    Article Title: ADAM17-dependent proteolysis of L-selectin promotes early clonal expansion of cytotoxic T cells
    Article Snippet: .. Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552), were sequentially transduced with lentiviral particles isolated from transiently transfected 293 T cells to express an HIV-1 Gag SLYNTVATL-specific TCR (868) and either wildtype L-selectin or shedding-resistant ∆M-N human L-selectin . .. Full-length and ∆M-N-L-selectin were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent), and the PCR product was cleaved with BamHI and XhoI before ligation into BamHI-linearized pSxW using In-fusion (Clontech).

    Isolation:

    Article Title: ADAM17-dependent proteolysis of L-selectin promotes early clonal expansion of cytotoxic T cells
    Article Snippet: .. Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552), were sequentially transduced with lentiviral particles isolated from transiently transfected 293 T cells to express an HIV-1 Gag SLYNTVATL-specific TCR (868) and either wildtype L-selectin or shedding-resistant ∆M-N human L-selectin . .. Full-length and ∆M-N-L-selectin were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent), and the PCR product was cleaved with BamHI and XhoI before ligation into BamHI-linearized pSxW using In-fusion (Clontech).

    Transfection:

    Article Title: ADAM17-dependent proteolysis of L-selectin promotes early clonal expansion of cytotoxic T cells
    Article Snippet: .. Human L-selectin negative MOLT-3 acute lymphoblastoid T-cells (ATCC CRL-1552), were sequentially transduced with lentiviral particles isolated from transiently transfected 293 T cells to express an HIV-1 Gag SLYNTVATL-specific TCR (868) and either wildtype L-selectin or shedding-resistant ∆M-N human L-selectin . .. Full-length and ∆M-N-L-selectin were amplified using PfuUltra II Fusion HS DNA Polymerase (Agilent), and the PCR product was cleaved with BamHI and XhoI before ligation into BamHI-linearized pSxW using In-fusion (Clontech).



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